GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
Glyceraldehyde-3-phosphate Dehydrogenase
CAS: 9001-50-7
Molecular Formula: C3H7O6P
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Names and Identifiers
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Physico-chemical Properties
| Molecular Formula | C3H7O6P |
| Molar Mass | 170.057841 |
| Appearance | suspension |
| Color | white |
| Storage Condition | -20°C |
| MDL | MFCD00131203 |
| Physical and Chemical Properties | White crystalline substance suspended in 3.2mol/L ammonium sulfate solution (containing 0.1mmol/L EDTA). Stability: under the condition of 5 ℃, the suspension can be stored for several months without changing its vitality. Regular replacement of new media can keep the activity unchanged for a long time. Freezing or thawing can deactivate it. Each molecule of glyceraldehyde-3-phosphate dehydrogenase contains 3.8-4.0 highly active cysteine residues, and can be combined with 4 molecules of coenzyme I (NAD) or reduced coenzyme I (NADH), enzyme reaction: D-glyceraldehyde-3-phosphate coenzyme I inorganic phosphorus_1, 3-diphosphate-D-glycerate reduction coenzyme I. |
| Use | It is used to determine the biochemical research of ATP glycerol -3-phosphate coupling reaction. It is commonly used as a diagnostic enzyme in clinic to directly determine the activity of phosphoglycerate kinase. Commonly used diagnostic enzymes. Determination of ATP glyceraldehyde-3 phosphate coupling reaction biochemical studies, and can be directly used to determine the activity of phosphoglycerate kinase. |
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Risk and Safety
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. |
| WGK Germany | 3 |
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Source
Rabbit muscle.
Last Update：2023-08-16 21:32:38
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Vitality
≥75 units/mg protein
Last Update：2023-08-16 21:32:38
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Definition of enzyme activity
One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.
Last Update：2023-08-16 21:32:38
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Preparation
CN201711376257.7 provides a glyceraldehyde 3-phosphate dehydrogenase and its polyclonal antibody. The glyceraldehyde 3-phosphate dehydrogenase derived from bacteria was obtained, and the glyceraldehyde 3-phosphate dehydrogenase has the characteristics of low temperature enzyme, and the polyclonal antibody of glyceraldehyde 3-phosphate dehydrogenase was prepared, which can accurately detect glyceraldehyde 3-phosphate dehydrogenase. One of the objects of the present invention is to provide an amino acid sequence of glyceraldehyde 3-phosphate dehydrogenase. The amino acid sequence of glyceraldehyde 3-phosphate dehydrogenase is SEQ ID NO. 1.
The second object of the present invention is to provide a base sequence encoding a glyceraldehyde-3-phosphate dehydrogenase gene; the base sequence of the glyceraldehyde-3-phosphate dehydrogenase gene is such as SEQ ID NO. 2. The third object of the present invention is to provide a method for preparing a polyclonal antibody of glyceraldehyde 3-phosphate dehydrogenase. Include the following steps:
1) Using glyceraldehyde 3-phosphate dehydrogenase with amino acid sequence as shown in SEQ ID No.1 as antigen, immunize mice to prepare antiserum;
2) Purified antiserum to obtain polyclonal antibody of glyceraldehyde 3-phosphate dehydrogenase. Further, the preparation method of glyceraldehyde 3-phosphate dehydrogenase is as follows: 1.1) using PCR technology to amplify SEQ ID NO. The base sequence shown in 2; 1.2) SEQ ID NO. The base sequence shown in 2 is cloned into the vector plasmid to construct the expression vector of glyceraldehyde 3-phosphate dehydrogenase; 1.3) The expression vector is transformed into the E. coli competent bacteria to obtain the recombinant bacteria, and the 3-phosphate glyceraldehyde dehydrogenase protein is obtained by expanding culture, inducing expression, collecting bacteria, crushing bacteria and purifying protein. Fig. 3 is a polyacrylamide gel electrophoresis identification chart of recombinant strain Ecoli BL21-pET21a-GPR expressing glyceraldehyde 3-phosphate dehydrogenase protein. In which lane m is protein marker; Lane 1 is glyceraldehyde 3-phosphate dehydrogenase protein;
Last Update：2024-04-10 22:29:15
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - Production method
muscle is used as raw material, KOH extraction, ammonium sulfate fractionation, Sephadex G-50 column chromatography and CM-Sephadex column chromatography, and finally ammonium sulfate precipitation and recrystallization are obtained.
Last Update：2024-04-10 22:29:15
Supplier List
Spot supply
Product Name: Glyceraldehyde-3-phosphate Dehydrogenase Visit Supplier Webpage Request for quotationCAS: 9001-50-7
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
Spot supply
Product Name: Glyceraldehyde-3-phosphate Dehydrogenase Visit Supplier Webpage Request for quotationCAS: 9001-50-7
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
View History